Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated.

We have previously shown that fully synthesized prepro-alpha-factor (pp alpha F), the precursor for the yeast pheromone alpha-factor, can be translocated posttranslationally across yeast rough microsomal (RM) membranes from a soluble, ribosome-free pool. We show here that this is not the case for translocation of pp alpha F across mammalian RM. Rather we found that a small amount of translocation of full-length pp alpha F is observed, but is solely due to polypeptide chains that were still ribosome bound and covalently attached to tRNA, i.e., not terminated. In addition, both signal recognition particle (SRP) and SRP receptor are required, i.e., the same targeting machinery that is normally responsible for the coupling between protein synthesis and translocation. Thus, the molecular requirements for targeting are distinct from posttranslational translocation across yeast RM. As termination is generally regarded as part of translation, the translocation of full-length pp alpha F across mammalian RM does not occur "posttranslationally," albeit independent of elongation. Most other proteins for which posttranslational translocation across mammalian RM was previously claimed fall into the same category in that ribosome attachment as peptidyl-tRNA is required. To clearly separate these two distinct processes, we suggest that the term posttranslational be reserved for those processes that occur in the complete absence of the translational machinery. We propose the term "ribosome-coupled translocation" for the events described here

Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes.

Ribosomes synthesizing nascent secretory proteins are targeted to the membrane by the signal recognition particle (SRP), a small ribonucleoprotein that binds to the signal peptide as it emerges from the ribosome. SRP arrests further elongation, causing ribosomes to stack behind the arrested ribosome. Upon interaction of SRP with its receptor on the ER membrane, the translation arrest is released and the ribosome becomes bound to the ER membrane. We have examined the distribution of unattached and membrane-bound ribosomes during the translation of mRNAs encoding two secretory proteins, bovine preprolactin and rat preproinsulin I. We find that the enhancement of ribosome stacking that occurs when SRP arrests translation of these proteins is relaxed in the presence of microsomal membranes. We also demonstrate that two previously described populations of membrane-associated ribosomes, distinguished by their sensitivity to high salt or EDTA extraction, correspond to ribosomes that have synthesized differing lengths of the nascent polypeptide. This analysis has revealed that nascent chain insertion into the membrane begins at distinct points for different presecretory proteins

Incorporation in vivo of 14C-Labelled Amino Acids into the Proteins of Mitochondrial Ribosomes from Neurospora crassa Sensitive to Cycloheximide and Insensitive to Chloramphenicol

Radioactive amino acids were incorporated in vivo into Neurospora crassa cells, and the mitochondrial ribosomes were isolated. The incorporation of radioactivity into the proteins of these ribosomes was inhibited by cycloheximide, but not by chloramphenicol. It is therefore concluded that these proteins are synthesized on the cycloheximide sensitive and chloramphenicol insensitive cytoplasmic ribosomes

Neutrino factory in stages: Low energy, high energy, off-axis

We discuss neutrino oscillation physics with a neutrino factory in stages, including the possibility of upgrading the muon energy within the same program. We point out that a detector designed for the low energy neutrino factory may be used off-axis in a high energy neutrino factory beam. We include the re-optimization of the experiment depending on the value of theta_13 found. As upgrade options, we consider muon energy, additional baselines, a detector mass upgrade, an off-axis detector, and the platinum (muon to electron neutrino) channels. In addition, we test the impact of Daya Bay data on the optimization. We find that for large theta_13 (theta_13 discovered by the next generation of experiments), a low energy neutrino factory might be the most plausible minimal version to test the unknown parameters. However, if a higher muon energy is needed for new physics searches, a high energy version including an off-axis detector may be an interesting alternative. For small theta_13 (theta_13 not discovered by the next generation), a plausible program could start with a low energy neutrino factory, followed by energy upgrade, and then baseline or detector mass upgrade, depending on the outcome of the earlier phases.Comment: 23 pages, 10 (color) figures. Minor clarifications and changes. Final version to appear in PR

Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth.

We have isolated and sequenced genes from Saccharomyces cerevisiae (SRP54SC) and Schizosaccharomyces pombe (SRP54sp) encoding proteins homologous to both the 54-kD protein subunit (SRP54mam) of the mammalian signal recognition particle (SRP) and the product of a gene of unknown function in Escherichia coli, ffh (Römisch, K., J. Webb, J. Herz, S. Prehn, R. Frank, M. Vingron, and B. Dobberstein. 1989. Nature (Lond.). 340:478-482; Bernstein H. D., M. A. Poritz, K. Strub, P. J. Hoben, S. Brenner, P. Walter. 1989. Nature (Lond.). 340:482-486). To accomplish this we took advantage of short stretches of conserved sequence between ffh and SRP54mam and used the polymerase chain reaction (PCR) to amplify fragments of the homologous yeast genes. The DNA sequences predict proteins for SRP54sc and SRP54sp that are 47% and 52% identical to SRP54mam, respectively. Like SRP54mam and ffh, both predicted yeast proteins contain a GTP binding consensus sequence in their NH2-terminal half (G-domain), and methionine-rich sequences in their COOH-terminal half (M-domain). In contrast to SRP54mam and ffh the yeast proteins contain additional Met-rich sequences inserted at the COOH-terminal portion of the M-domain. SRP54sp contains a 480-nucleotide intron located 78 nucleotides from the 5' end of the open reading frame. Although the function of the yeast homologues is unknown, gene disruption experiments in S. cerevisiae show that the gene is essential for growth. The identification of SRP54sc and SRP54sp provides the first evidence for SRP related proteins in yeast

On the Pressure Broadening in the Gamma Bands of Nitric Oxide

A quantitative investigation of the pressure broadening in the γ(0,0) and γ(1,0) bands of nitric acid established that the pressure effect is not abnormal as has sometimes been supposed and that the collision diameter of the excited NO molecule is approximately 3.8 Å